Hyperbaric oxygen therapy provides neuroprotection following spinal cord injury in a rat model

International Journal of Clinical and Experimental Pathology

Huiqiang Chen¹  Jing Gu¹  Wei Zhang¹  Lei Xue¹  Xiaodong Chen¹  Huai Huang¹  Qibiao Weng¹  Huangyi Liao¹  Xu Zhang¹  Shuilin Ye¹ 

关键词: Hyperbaric oxygen;    spinal cord injury;    rat;    nitric oxide;    inducible nitric oxide synthase;   

DOI  :  

学科分类：生理学与病理学

来源: e-Century Publishing Corporation

【 摘 要 】

Objective: To investigate the effect of hyperbaric oxygen therapy (HBOT) on the iNOS mRNA-iNOS-NO signaling pathway and neurofunction protected in a rat spinal cord injury model. Methods: A total of 36 Sprague-Dawley rats were randomly divided into 3 groups: control group (n=12), SCI group (n=12) and SCI + HBOT group (n=12). SCI + HBOT group In the SCI group and SCI + HBOT groups, SCI was performed on rats. In the SCI + HBOT group, rats with SCI underwent HBO treatment 30 min after SCI for 24 sessions. After HBO therapy, measurement of motor evoked potential (MEP), Basso, Beattie, Bresnahan (BBB) scoring and pathological examination were done. RT-PCR and immunohistochemistry were employed to detect the mRNA and protein expression of iNOS, respectively. Diazo colorimetry was performed to detect the serum NO content. Results: The mRNA and protein expression of iNOS in the spinal cord and the serum NO content were markedly increased in the SCI group as compared to the control group (P<0.05). However, the mRNA and protein expression of iNOS and the serum NO content were dramatically reduced in the SCI + HBOT group as compared to the SCI group (P<0.05). Conclusion: HBO therapy can promote the neuroprotection following SCI, which may be related to the effect of HBO on the iNOS mRNA-iNOS-NO signaling pathway.

【 授权许可】

Unknown