Effective compounds screening from Rabdosia serra (Maxim) Hara against HBV and tumor in vitro

International Journal of Clinical and Experimental Medicine

Hongyuan Zhu¹  Xiuzhen Zhang¹  Cheng Chen¹  Jingfeng Zhao¹  Yang Chen¹  Yuxiang Chen¹  Yiyun Xiao¹ 

关键词: Rabdosia serra (Maxim.) Hara;    effective compounds;    anti-HBV;    anti-tumor;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

The aim of this study was to screen and investigate the anti-HBV and anti-tumor activities of separated compounds from Rabdosia serra (Maxim.) Hara to lay the basis for further isolate active entity. Three kinds of extractions from Rabdosia serra using different solvents (petroleum ether, acetidin, butyl alcohol) were prepared and used to analyze their anti-HBV activity in HepG2.2.15 cells for further separation. The cytotoxicity of each extraction was tested by MTT assay, the levels of HBsAg, HBeAg and HBV DNA in supernatants from HepG2.2.15 cells were detected by ELISA and real-time quantitative polymerase chain reaction (PCR). Then, the most effective extraction was further separated, the anti-HBV activities of separated compounds were also tested by MTT and ELISA, and three compounds with highest cytotoxicity were selected to further identify their anti-tumor activities on MCF-7, BGC-823 and HepG2 cells. Acetidin extraction C₂ had the most effective anti-HBV activity that was used to be further separated, it led to statistically significant reduction in HBsAg and HBeAg secretion and HBV DNA. The separation of C₂ resulted in 14 compounds, A₃ and A₅ markedly inhibited HBsAg secretion, while A₉ inhibited HBeAg secretion in a dose-dependent manner with higher TI comparing with C₂. A₆, A₇, A₁₁ had different anti-tumor activity against different tumor cells. These data showed that the extraction and their separated effective compounds had strong inhibitory effect on HBV replication so as to have anti-HBV activity, and further separation and purification could enhance anti-HBV activity. Meanwhile, some compounds have high cytotoxicities on different tumor cells. Our study could provide a theoretical basis for the next clinical use and the development of potential and efficient drugs for HBV and tumor therapy from Rabdosia serra.

【 授权许可】

Unknown