Effects of intrathecal anesthesia with different concentrations and doses on spinal cord, nerve roots and cerebrospinal fluid in dogs

International Journal of Clinical and Experimental Medicine

Na Lv¹  Yongjun Su¹  Dawei Yang¹  Yang Liu¹  Jianping Zhang¹  Jianrong Guo¹ 

关键词: Amides;    intrathecal anesthesia;    spinal cord;    nerve root;    ultrastructure;    free radical;    Ca2+;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Objective: To investigate the effects of intrathecal anesthesia with bupivacaine, levobupivacaine and ropivacaine hydrochloride at different doses on the spinal cord, nerve roots and cerebrospinal fluid (CSF) in dogs. Methods: Forty-two mongrel dogs were randomly divided into normal saline group (C; 2 ml), 0.5% (B₁) and 0.75% (B₂) bupivacaine hydrochloride groups (2 ml), 0.5% (L₁) and 0.75% (L₂) levobupivacaine hydrochloride group (2 ml), 0.5% (R₁) and 0.75% (R₂) ropivacaine hydrochloride group (2 ml), and drugs were intrathecally injected. Results: The contents of Ca²⁺ and MDA and SOD activity of the spinal cord were comparable among groups (P > 0.05). In Groups B₁, L₁ and R₁, the neuronal cytoplasm of spinal tissues was basically normal, the majority of mitochondria and endoplasmic reticulum had complete structure, and the lamellar structure of modulated fibers was nearly normal. In Groups B₂, L₂ and R₂, a small amount of mitochondrial vacuolar degeneration was found in the neuronal cytoplasm of spinal cord, but their structures were basically normal; the neural tissues exhibited focal mild edema, and most of the lamellar structure of modulated fibers and Schwann cells were nearly normal except for loose structure in several fibers and cells. Conclusion: When compared with 0.75% anesthetics for local anesthesia, the early adverse effects on the ultrastructure of the spinal cord and nerve root reduce after focal anesthesia with 0.5% anesthetics.

【 授权许可】

Unknown