JAK kinases are required for the bacterial RNA and poly I:C induced tyrosine phosphorylation of PKR

International Journal of Clinical and Experimental Medicine

Farag Bleiblo¹  Joseph E Parrillo¹  Aseem Kumar¹  Chilakamarti V Ramana¹  Danielle Brabant¹  Mazen Saleh¹  TC Tai¹  Paul Michael¹  Anand Kumar¹ 

关键词: PKR;    JAKS;    tyrosine phosphorylation;    bacterial RNA;    innate immunity;   

DOI  :  

学科分类：医学（综合）

来源: e-Century Publishing Corporation

【 摘 要 】

Discriminating the molecular patterns associated with RNA is central to innate immunity. The protein kinase PKR is a cytosolic sensor involved in the recognition of viral dsRNA and triggering interferon-induced signaling. Here, we identified bacterial RNA as a novel distinct pattern recognized by PKR. We show that the tyrosine phosphorylation of PKR induced by either bacterial RNA or poly I:C is impaired in mutant cells lacking TYK2, JAK1, or JAK2 kinases. PKR was found to be a direct substrate for the activated JAKs. Our results indicated that the double-stranded structures of bacterial RNA are required to fully activate PKR. These results suggest that bacterial RNA signaling is analogous in some respects to that of viral RNA and interferons and may have implications in bacterial immunity.

【 授权许可】

Unknown