Impact of burn priming on immune and metabolic functions of whole Liver in a rat cecal ligation and puncture model

International Journal of Burns and Trauma

John SA Mattick¹  Francois Berthiaume¹  Ioannis P Androulakis¹  Marianthi G Ierapetritou¹  Mehmet A Orman¹  Stephen C Gale¹  Qian Yang¹ 

关键词: Sepsis;    burns;    microarray analysis;    RT-PCR analysis;    liver;   

DOI  :  

学科分类：外科医学

来源: e-Century Publishing Corporation

【 摘 要 】

Previously, we have shown that systemic insults in single injury models produced immunosuppressive effects in burn, and a strong acute phase response in sepsis through hepatic gene expression. In order to investigate the implications of these effects on a combined injury, a double hit model was explored to mimic the progression of clinical burn-sepsis. Rodents were subjected to a 20% total body surface area (TSA) full-thickness burn injury, and 48 hours later underwent cecal ligation and puncture (CLP) to induce sepsis. Pathways related to innate immune signaling through cytokines and NF-KB were co regulated with xenobiotic metabolism genes and acute phase protein genes, and that these genes were suppressed early, and then activated. Furthermore, we were able to identify that, in addition to amino acid metabolism, pyruvate metabolism, fatty acid metabolism and NRF-2 mediated oxidative stress genes were down regulated over the time course. Overall, these observed trends within the double hit burn-sepsis model represent unique immune and metabolic pathways and dynamics not found in either injury, including an early suppression followed by overreaction of pro inflammatory mediators, and an increase in amino acid metabolism at the expense of central carbon pathways.

【 授权许可】

Unknown