Investigation of TEL/AML1 and BCR/ABL genes fusion in Acute lymphoblastic leukemia (ALL) Patients and Follow-up Study in 25 Bone Marrow Transplanted (BMT) Patients Using Interphase Fluorescence In Situ Hybridization (FISH).

lranian journal of Clinical Infectious Diseases

AM Aghazadeh¹  F Aboualsoltani²  SA Rahmani⁴  M Zamani⁵  P Mehdipour⁶  M Izadyar³ 

[1] Assistant Professor of Biostatistics, Tabriz University of Medical Sciences.;General Practitioner, Tabriz University of Medical Sciences,;Associate Professor of Hematology and Oncology, Tehran University of Medical Sciences,;Assistant Professor of Medical Genetics, Tabriz University of Medical Sciences;Assistant Professor of Medical Genetics, Tehran University of Medical Sciences,;Professor of Medical Genetics, Tehran University of Medical Sciences,

关键词: ALL;    BMT;    FISH;   

DOI  :  10.17795/semj20513

学科分类：基础医学

来源: Shiraz University of Medical Sciences

【 摘 要 】

Introduction::BCR/ABLfusionsinhematopoeiticcellsareknowntoinduceresistancetoapoptosisandcellchangesinresponsetocell-cellandcell-matrixinteractions,ontheotherhand,patientswithTEL/AML1genefusionsresponddifferentlytotreatment,dependingontherapeuticprotocols.Aims::Weconductedaprospectivecohortstudytoinvestigatehowthesetranslocationsaffectaperson’squalityoflife,andtoevaluatetheirresponsestobonemarrowtransplantationtherapy.MethodsandMaterials::TEL,AML1,ABLandBCRprobeswereappliedtocellsduringinterphase,usingcytogenetictechniquesandFISHanalysistoobtainthekaryotypeof100patients,whichincludedgenesinvolvedinfusion,signaldistributions,age,sex,positivefamilialbackground,andresponsestotherapies.AfterBMTwasperformedin25patients,alloftheabovedatawascollectedonceagainandtheresultswerecompared.Results::Inourstudy,46%ofchildpatientsdemonstratedanabnormalFISHpattern(23%withfusedABL/AML1,3%withdeletion,7%withagaininTELgene,and3%and10%withdeletionandagaininAML1genes,respectively.Inadults,27%hadanabnormalFISHpattern,while3%hadfusedTEL/AML1genesandotherabnormalities,aswasevidentinchildren.Againingenecopyoccuredtwiceasoftenasalossingenecopy,exceptforchildALLpatientswitht(12;21),wherein58%ofcases,lostTELgenechildrenwitht(12;21)hadlongersurvivalperiods,whileadultswitht(9;22)hadshorterones.PostBMTrevealedthat65%ofBMcellskaryotypednormal,comparedto24%pre-BMT.WBCcountincreasedpositivelywiththeonsetofALL,althoughanincreaseinWBCcountdecreasedsurvivaltime.ArelationshipbetweenpositivefamilialbackgroundandALLwasalsoseen.Conclusion::FISHisthebettermethodfordiagnosinggeneticdisordersinALLpatientscomparedtoothermethods.

【 授权许可】

Unknown