Evaluation of Antioxidant Enzymes, Lipid Peroxidation and Proline Content as Selection Criteria for Grain Yield under Water Deficit Stress in Barley

Journal of Applied Biological Sciences

Armin Saed-MOUCHESHI¹  Mohammad Bagher ZAHEDI¹  Hooman RAZI¹ 

关键词: Hordeum vulgare;    Superoxide dismutase;    Catalase;    Protein content;    MDA;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Screeningplantgeneticresourcesunderwaterdeficitenvironmentusingreliableselectioncriteriaisanessentialstepinbreedingprogramstowardsdroughttoleranceimprovement.Inthisgreenhouseexperiment,thetwenty-fivebarley(HordeumvulgareL.)cultivarsweresubjectedtowell-wateredandwaterdeficitstressconditionsinordertomeasurechangesofantioxidantenzymesactivities,malondialdehyde(MDA),prolineandproteincontentsinresponsetowaterdeficitstressandtoevaluatethesignificanceofthebiochemicaltraitsasselectioncriteriaforimprovementofgrainyield.Significantvariationswereobservedamongthebarleycultivarsforgrainyieldaswellasthebiochemicaltraitsincludingtheantioxidantenzymes,MDA,proteinandprolinecontents.WaterdeficitstresscausedasignificantincreaseinMDA,prolineandproteincontentsaswellastheantioxidantenzymesactivitiesincludingcatalase,peroxidase,superoxidedismutaseandascorbicperoxidase.Theresultsofcorrelationcoefficientsandregressionmodelingshowedthattherelationshipsbetweengrainyieldandbiochemicaltraitsweresomewhatinfluencedbywaterregimes.Theresultssuggestedthathighprolinecontentalongwithlowproteincontentcanbeusedasthecriteriaforselectionofhigh-yieldbarleycultivarsunderwell-wateredconditions.Catalaseandsuperoxidedismutaseactivitiesaswellasprolinecontentwereknownastheselectioncriteriawithsignificantcontributionstograinyieldofbarleyunderwaterdeficitconditions.Catalaseandsuperoxidedismutaseactivitiesweresignificantlypositivelyrelatedtograinyield,whileprolinecontenthadanegativerelationshipwithgrainyieldunderwaterdeficitconditions.

【 授权许可】

Unknown