Multiple pathways are responsible for Anti-inflammatory and Cardiovascular activities of Hordeum vulgare L.

Journal of Translational Medicine

Muhammad Zia-Ul-Haq¹  Marius Moga⁴  Hawa ZE Jaafar²  Abrar Hussain⁵  Nafisa Batool Tahir⁷  Qazi Tahir Uddin³  Nurolaini Kifli⁸  Sagheer Ahmed⁶  Saima Gul⁸ 

[1] The Patent Office, Karachi, Pakistan;Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, Selangor, 43400, Malaysia;Department of Surgery, Khyber Medical University Institute of Medical Sciences, Kohat 26000, Pakistan;Department of Medical and Surgical Specialities, Transilvania University of Brasov, Brasov, Romania;Department of Biotechnology & Informtics, Baluchistan University of Information Technology, Engineering & Management Sciences, Quetta, Quetta-87300, Pakistan;Department of Pharmacy, Kohat University of Science & Technology, Kohat 26000, Pakistan;Department of Medicine, Khyber Medical University Institute of Medical Sciences, Kohat 26000, Pakistan;PAPRSB Institute of Health Sciences, University Brunei Darussalam, Bander Seri Begawan, Gadong BE1410, Brunei Darussalam

关键词: Glutathione peroxidase;    Superoxide dismutase;    Lipoxygenase;    Cyclooxygenase;    Platelets;    Hordeum vulgare;    Inflammation;   

Others  :  1147076
DOI  :  10.1186/s12967-014-0316-9

 received in 2014-08-14, accepted in 2014-10-28,  发布年份 2014

【 摘 要 】

Background

Hordeum vulgare L. (HV or barley) is used by traditional healers to treat various inflammatory and cardiovascular diseases, without the knowledge of pharmacologic rationale behind its actions. This study was designed to explore the potential scientific mechanism(s) that could explain the use of Hordeum vulgare in traditional medicine as a treatment for various inflammatory and cardiovascular diseases.

Methods

A crude extract and its three fractions were prepared from HV and screened for the inhibition of platelet aggregation and various metabolites of cyclooxygenase (COX), lipoxygenase (LOX) pathways of arachidonic acid (AA) metabolism as well as for its effects on certain antioxidant enzymes. Platelet aggregation was monitored using turbidometric principle, AA metabolism through radioimmunoassay and antioxidant enzymes by commercial kits using spectrophotometer.

Results

Results show that HV exhibited activities against all human platelet agonists used except adenine diphosphate, and inhibited both COX and LOX pathways of AA metabolism. It also elevated the activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx). However, these activities were distributed in various fractions of HV. Aqueous fraction was most potent in elevating SOD activity; chloroform fraction had concentrated compounds responsible for COX inhibition while n-hexane seems to possess compounds responsible for LOX inhibition as well as the only fraction enhancing the activity of GPx.

Conclusions

These results suggest the likely mechanisms responsible for observed anti-inflammatory and cardiovascular effects of HV in traditional medicine.

【 授权许可】

   
2014 Gul et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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