Comparison of the dicentric yield observed in metaphase cells obtained using only Colcemid or Colcemid and Calyculin-A

Journal of Applied Biological Sciences

Lotfi LOUHIBI¹  Nadhira Saidi-MEHTAR¹  Joan Francesc BARQUINERO¹  Abdelah BOUJEMAA¹  Ahlem-Nora Messal-DJELTI¹  Leonardo BARRIOS¹  Maria Rosa CABALLIN¹ 

关键词: Biodosimetry;    Dicentrics;    Premature chromosome condensation;    Calyculin-A;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

TheobjectiveofBiologicalDosimetryisitsapplicabilityinradioprotection,quantifyingthedosereceivedinindividualswhohavebeenexposedtoionizingradiation.Forthisraison,theanalysisofthefrequencyofchromosomeaberrationspresentinfirstdivisionmetaphasesfromstimulatedlymphocytesisacceptedasbeingthereliable.However,inproliferatingcellsthereareseveralcheckpointsthatpreventdamagedcellstopassthrough,andheavilydamagedcellsshowanimpossibilitytoreachmitosis.ConcretelythechemicallyinducedprematurechromosomecondensationbyCalyculin-AincombinationwithColcemiditispossibletoscoreinthesameslidecellsthatwereininterphaseandinmetaphase.TheaimistodetermineifCalyculin-Ahasanyeffectinthedicentricyieldobservedinmetaphasecells.Peripheralbloodsampleswereirradiatedat1,4,and10Gyofγ-raysandculturedfor48hours.Colcemidwasadded24or2hoursbeforeharvesting,andforcomparisonscultureswerealsotreatedwithofCalyculin-Aatthelasthour.ForthethreedosestheproportionofMcellswasalwayshigherafter24hoursinpresenceofColcemidthanafter2hours.Whenthefrequenciesofdicentricswerecompared,therewerenomajordifferencesbetweencultureswhereonlyColcemidwasused(2or24hours)andthosewheretheCalyculin-Atreatmentwasalsodone.TheresultsindicatethatwhenPCC-CAisusedifmetaphasecellsareconsideredsimilardose-effectcurveswillbeobtainedthanthoseobtainedusingtheconventionalmitoticarrestwithColcemid.

【 授权许可】

Unknown