Formulation of Therapeutic Phage Cocktails to Human Isolates of Salmonella enterica Serovar enteritidis

Journal of Applied Biological Sciences

Ahmed Sahib ABDULAMIR¹  Rafal Nazar TAHA¹ 

关键词: Salmonella;    bacteriophage;    phage cocktails;    phage therapy;    alternative medicine.;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Background:Antibioticshaverevolutionizedmedicineinmanyrespects.Regrettably,theuseofthesewonderdrugshasbeenaccompaniedbytherapidappearanceofresistantstrains.Salmonellaserotypeenteritidisisoneoftheimportantcausesofhumanillness;resistanceofthesebacteriatoantibioticspreventsthesuccessofpropertherapy.Bacteriophagecouldbeusedasanalternativetoconventionalantibiotictherapy.Objectives:ToformulateIraqitherapeuticphagecocktailscomposedof,in-laboptimized,highlyspecificphagesagainstSalmonellaenteritidis,toevaluateandmeasuretherateofbacterialresistancetotheformulatedphagecocktails.MaterialsandMethods:Salmonellaenteritidisbacteriawereisolatedfromhumansamplesandtestedforantibioticssusceptibility,thenspecificlyticphagesforthesebacteriawereisolatedbyspotlysisassay.Afterwards,phagetitrationwasconductedandexaminationofphagecharacteristicswasdonebytoplayerplaqueassay.Testingofbacterialresistancetotheformulatedphagescocktailswasdetermined.Results:SevenphageswereisolatedforSalmonella,eachphagewasuniqueinitscharacteristicsincludingclarity,shape,size,andedgeofplaquesformedandtheirtiter.Conclusion:Phagecocktailsaremoreeffectivethanindividualphagesastheyshowclearerplaquesandlessresistancerate.Thisstudyconcludesthatphagetherapyisaverypromisingalternativetotraditionalantibiotictherapy

【 授权许可】

Unknown