Genomics and Transcriptomics Analysis of Cu Accumulator Plant Brassica nigra L.

Journal of Applied Biological Sciences

Abdul Razaque MEMON¹  Emina ZAHIROVIC¹ 

关键词: Brassica nigra;    Genomics;    transcriptomics;    microRNAs;    Cu accumulator;    phytoremediation;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

ThegenusBrassicacontainsawiderangeofdiploidandamphipolyploidspeciesincludingsomeoftheeconomicallyhighvaluablevegetablesandoilseedcropsusedworldwide.ThemajorindustrialandfoodcropsinBrassicaaretheclosestrelativestothemodelplantArabidopsisthaliana,andhencearemajorbeneficiariesfromthevastdataofgenomicsandmoleculargeneticsavailableinthedatabaseofArabidopsisthaliana.ExtensivegeneticandmolecularanalyseshavebeenundertakenforthesixcultivatedBrassicaspecies.ThefourcloselyrelatedcropspeciesB.rapa(AA,2n=20),B.juncea(AABB,2n=36),B.napus(AACC,2n=38),andB.carinata(BBCC,2n=34)provideabout12%oftheworldwideedibleoilsupply.TheothertwospeciesB.nigra(BB,2n=16)andB.oleracea(CC,2n=18)providemanyvegetablesforhealthyhumandiethavingavaluablesourceofdietaryfiber,vitaminCandotheranticancercompounds.ThecomparativemappingbetweenArabidopsisthalianaandBrassicacropspecies,coupledwiththebaseknowledgeofmutationbasedfunctionalanalysisinArabidopsisthalianaandQTLmappingincropBrassicas,couldgreatlycontributetowardsabetterunderstandingofthegeneticarchitecturefortheconservedaswellastheevolvedtraitsofagronomicvalueintheBrassicaceae.Brassicanigrahasthesecondsmallestgenomesize(~632Mbp)amongthesixcultivatedspeciesofBrassica.Brassicaspeciesarewellknownasmetalaccumulatorsandsomeofthemarebeingusedforphytoremediationincontaminatedsoils.Approximately25%ofthedocumentedmetalhyperaccumulatingspeciesare,likeA.thaliana,membersoftheBrassicaceae.ThesupermetalaccumulatingcapacityofArabidopsishalleriandNoccaeacaerulescens(previouslyThlaspicaerulescens)havebeenwelldocumented.Becauseoftheirslowgrowthandlowbiomass,otherfast-growingandhighbiomassbrassicacropplants,forexampleBrassicajunceaandBrassicanigrahavebeenevaluatedfortheirabilitytohyperaccumulatemetalsfromcontaminatedsoils.TheDiyabekerecotypeofB.nigracollectedfromsoutheasternpartofTurkeywasfoundtobehyperaccumulatorofCu.WecarriedoutthecomparativetranscriptomeanalysisinordertofindouttheexpressionlevelofmetalinducedgenesandtranscriptomechangesbothinlowandhighCutreatedplants.Microarrayanalysisshowedthatsomeofthegeneswerehighlyexpressed(severalhundredfold)withCutreatedplantscomparedtocontrol.OurmicroarraydatausingAffymetrixGeneChipArabidopsisGenomeArray(ATH1-121501Genechip)indicatethatpossiblyseveralgenesincludingthegenesinglutathionepathway,metalATPaseandABCtransportersareinvolvedinmetaltolerancesinthisecotype.InthiscommunicationtheuseofmoleculartoolsandtheexploitationofArabidopsisknowledgewillbepresentedindetail.

【 授权许可】

Unknown