Relationships Between Levels of Reactive Oxygen Species in Spermatozoa and GSTM1 Polymorphism in Infertile Men

Journal of Applied Biological Sciences

Gönül GÜROL¹  İlhan ONARAN¹  Bülent ALICI¹  Ali Rıza KIZILER¹  Fatma Behice CİNEMRE¹  Birsen AYDEMİR¹ 

关键词: GSTM1 polymorphism;    Reactive Oxygen Species;    Spermatozoa;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

ThepurposeofthisstudywastoexaminerelationshipsbetweenglutathioneS-transferaseMu-1(GSTM1)genepolymorphismandthesusceptibilityofspermatozoafrompatientswithidiopathicinfertilitytooxidativestress.Fifty-twomenwithidiopathicinfertilityand60healthyfertilemenwererecruitedtothisstudy.GSTM1genepolymorphismwasdeterminedbypolymerasechainreaction(PCR)andboththeinfertileandcontrolindividualsweredividedintoGSTM1nullandGSTM1positivegroupsaccordingtotheirGSTM1genestructure.ROSformationinspermatozoawasfollowedbychemiluminescenceofluminol-fluoresceine-enhancedsystem.Wecomparedreactiveoxygenspecies(ROS)generationinspermatozoafrominfertilepatientsandcontrolswithrespecttoGSTM1genotype.SignificantlyhigherlevelsofROSwerefoundinidiopathicinfertilemenwiththeGSTM1nullgenotypecomparedwiththosewiththeGSTM1positivegenotype.TherewasnosignificantdifferenceingenotypedistributionfortheGSTM1variantbetweentheidiopathicinfertilesubjectsandfertilesubjects.OurresultssuggestthatthesusceptibilityofspermatozoatooxidativestressissignificantlygreaterinidiopathicinfertilemenwiththeGSTM1nullgenotypecomparedwiththosepossessingthegene.Therefore,inpatientswithidiopathicinfertility,GSTM1polymorphismmightbeanimportantsourceofvariationinsusceptibilityofspermatozoatooxidativedamage.

【 授权许可】

Unknown