A high‐conductance voltage‐dependent multistate Ca2+ channel found in sea urchin and mouse spermatozoa

FEBS Letters

Labarca, P.¹  Beltrán, C.²  Darszon, A.²  Liévano, A.² 

[1] Centro de Estudios Científicos de Santiago y Universidad de Chile, Chile;Dept. Bioquímica, Instituto de Biotecnología, Universidad National Autónoma de México, Mexico

关键词: Spermatozoa;    Sperm ion channel;    Ca2+ channel;   

DOI  :  10.1016/0014-5793(94)80109-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ion fluxes through poorly understood channel-mediated mechanisms participate in the interaction between spermatozoa and egg. Previously, we reported the characterization in planar bilayers of a high conductance Ca²⁺-selective, voltage-dependent multistate channel from S. purpuratus sea urchin sperm plasma membranes [14]. Here we show that this ion channel can be directly transferred to planar lipid bilayers upon sperm addition, from sea urchin [S. purpuratus and L. pictus) and from mouse. We found that spermatozoa from these species posses a conspicuous Ca²⁺-selective, high conductance, multi-state, voltage-dependent channel, which displays similar voltage dependence and equal PBa²⁺/P_K ⁺ ~4 in the three species. The presence of this Ca²⁺ channel in such diverse species suggests it plays a relevant role in sperm physiology. The high sensitivity of planar bilayers to detect single ion channels can now be used to study ion channel regulation and gamete interaction.

【 授权许可】

Unknown