The Determination of Self-Compabilitiy Status of Thermopsis turcica Through Histological Analysis

Journal of Applied Biological Sciences

Selim CETINER¹  Dilek TEKDAL¹ 

关键词: Hybridization;    pollen grain germination;    self-compatible;    Thermopsis turcica;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

ThermopsisturcicaKitTan,VuralKucukoduk(Fabaceae)isarareandendemicTurkishspeciesgrownaroundAksehirLakeandEberLakeofthesouthernareasofCentralAnatolia.T.turcicahasagynoeciumof2-4functionalcarpels.ThischaracteristicofT.turcicaisuniqueinthesubfamilyPapilioideaeofFabaceae.Althoughthisgenotypeisvaluableasfemaleplants,todatethereisnoconsiderationgiventothefertilizationbiologyofT.turcica.Inthepresentstudy,2populationsofspeciesT.turcica(EberandAksehirpopulations)wereused.SelfingandreciprocalcrossesbetweentwopopulationsofT.turcicawereperformedatNezahatGokyigitBotanicalGardenduringthepollinationperiodbetweenMayandJune2012.Pistilsampleswerecollectedfromthe1thto10thdayofthepollinationwithoutdamagingthepopulation.AllpistilscollectedwerefixedinFPA-70solutionandstoredat+4°Cuntilmicroscopicobservations.Pistilsampleswerestainedwithanilineblue.Afterstaining,pistilswerecutintotwoparts(stigmawithstyleandovary)andwerefurthercutlongitudinally,splitintotwoparts.Allsampleswereobservedunderafluorescentmicroscope.Inallthirdday-oldsamplesofT.turcica(bothofself-andreciprocalcrossedsamples),ovulefertilizationwasobserved.Basedonhistologicalanalysis’sresults,allsamplesofT.turcicahavebeenclearlyidentifiedasself-compatible.

【 授权许可】

Unknown