Investigation on Cryptosporidium spp. in water samples collected from River Melet in Ordu by Loop Mediated Isothermal Amplification (LAMP)

Journal of Applied Biological Sciences

Zeynep KOLOREN¹  Elif DEMİREL¹ 

关键词: Cryptosporidium;    LAMP;    River Melet;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Cryptosporidiumspp.wereidentifiedinwatersamplescollectedfromRiverMeletinOrduProvincebyLoopMediatedIsothermalAmplification(LAMP).ThewatersamplesareregularlytakeneverymonthfromRiverMeletwheremixtheseapoint(M-1),nearsolidwastestoragearea(M-2),Ordu-Giresunonthehighwaybridgelocation(M-3),nearthecity'sindustry(M-4)andinthecityout(M-5)fromDecember,2010toNovember,2011.60watersampleswereflocculatedbyAluminumSulfateandtheywereusedforthecountingofCryptosporidiumsp.oocystsbyModifiedAcid-Fast(MAF).Thehighestnumberofsampleswerepurifiedbysucrose-gradientandDNAwereextractedforLAMPassay.ThenumberofoocycstsdecreasedintheformofM-3,M-4,M-2,M-1veM-5,respectivelyinfivestationsofRiverMelet.Thesixwatersamplesfrompersite,whichisthehighestnumbersofoocystsbyMAF,wereanalyzedbyLAMPandtheywerefoundpositive.Orduhasrichwaterresources,but,theavailabilityofthemhavebeenreducedbecauseofthewastewatersdischargeofriverandseadirectlywithoutprocessing.Parasitesmovetorivers,lakesandstreamsbyrainfallinthisarea.Thereby,thedrinking,agricultureandrecreationaluseofwaterisimportanttoidentifypotentialriskfactorsfortheprotectionofthepublichealth.

【 授权许可】

Unknown