Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods

Journal of Applied Biological Sciences

Musa YAVUZ¹ 

关键词: In situ ruminal digestion;    bacterial protein;    purine;    neutral detergent fiber.;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Insituruminaldigestionprocedureisknownprocedureforestimatingruminaldigestion.Insituprocedurerequirescorrectionformicrobialcontaminations,andthepurinemethodhasbeenusedtocorrectmicrobialattachments.Theneutraldetergentfiber(NDF)methodhasbeenproposedasanalternativetothepurinemethod.ObjectivesofthisexperimentweretocomparepurineandNDFmethodformicrobialcorrection.Alfalfahay,bermudagrasshay,tallfescuehay,cornandsoybeanmealsampleswereselected.Chemicalanalysesofdrymatter(DM),crudeprotein(CP),NDF,Aciddetergentfiber(ADF),NDF-protein(NDIP),ADF-protein(ADIP),lignin,crudeoilandashweredeterminedonselectedsamples.Intheexperiment,sampleswereinsertedintotherumenofacannulatedcowfor6,12,24,72,and96htodetermineinsitudigestion.Theaveragemicrobialpurinetonitrogenratiowasdeterminedtobe11.43.InsitudigestedtallfescuemicrobialproteincorrectedCPvalueswithpurineandNDFmethodsweresimilarwithinallhours,except96h.OtherInsitudigestedsamplepurineprocedurehadsomeinterferenceduringsamplereadingandstandarderrorswerenotacceptablelevel.Therewerenotenoughdatatocomparebothmethodsforothersamples.

【 授权许可】

Unknown