Effects of Fe - Mg Limitations and Physiological Factors on P. aeruginosa Rhamnolipid

Journal of Applied Biological Sciences

Emir CANSUNAR¹  Tugba SUBAŞIOĞLU¹ 

关键词: Biosurfactant;    Pseudomonas aeruginosa;    rhamnolipid;    Fe limitation;    Mg limitation;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

AselectedPseudomonasaeruginosastrainwasgrownonthebasalmediumandinspectedforrhamnolipidbiosurfactantproduction.Inordertoincreasetherhamnolipidproduction,limitationsofbothmagnesiumandironelementsinthebasalmediumweretested.TheFeconcentrationsof0.05g/Landaboveinhibitedtheincreaseinbothcellgrowthandrhamnolipidproduction.MgconcentrationhadanegligibleeffectonrhamnolipidlevelsthanFe.WhileCarbon/Mgratioswerebetween400-2000,biosurfactantconcentrationincreasedandwhenCarbon/Feratiowasabove5000,biosurfactantproductionincreaseduntiltheratioreachedaboutto105.Inadditiontothenutrients,effectsofpHandtemperaturewerealsoinvestigated.Culturebrothswereincubatedatbetween20-40oC.Maximumrhamnolipidproductionandcellgrowthwereobservedat34.5oC.OptimumpHvaluesforrhamnolipidproductionwerefoundbetween6.5-7.0.

【 授权许可】

Unknown