Platelet lysate as an alternative growth factor source for in vitro expansion of mesenchymal stem cells

Cellular Therapy and Transplantation

Boris V. Afanasyev¹  Olga V. Burdanova¹  Ildar M. Barkhatov¹  Kirill V. Lepik¹ 

关键词: mesenchymal stem cells;    platelet lysate;    fetal bovine serum;   

DOI  :  10.3205/ctt-2010-No9-abstract65

学科分类：肿瘤学

来源: Universitaetsklinikum Hamburg - Eppendorf / University Medical Center Hamburg - Eppendorf

【 摘 要 】

Background:Duetoexistingperspectivesontheclinicalusageofbonemarrowmesenchymalstemcell(MSC)populations,themostcurrenttasksaredevelopmentoftheirisolation,expansion,andinfusionmethods.Presently,however,inmostprotocolsforMSCexpansion,theculturemediumincludestheadditionoffetalcowserum(FCS),whichisapotentialsourceofxenogenicantigensaswellinfectioncontamination.Humanplateletlysate(HPL)fromplateletenrichedplasmacouldbeconsideredasanalternativetoFCS.Objectives:TheaimofthestudywasthedevelopmentofHPLextractiontechniques,andevaluationofitsbiologicalactivityandpossibleuseasanalternativetoFCS.Methods:WeinvestigatedtheproliferativeactivityofMSCsinprimarycultureandduringlong-termcultivation.Theimmunophenotype,geneexpressionprofile,differentiationability,andhematopoiesissupportfunctionwerecomparedwithFCS-supplementedandserum-freemedium.Results:InassessingMSCs'colonyformationinprimaryculturethenumberofcolony-formingunitsoffibroblasts(CFU-f)washigherwhenHPLwasusedincomparisonwithFCS.DifferencesinMSCs'proliferationcapacitywasnotobservedduringlong-termcultivation.AlowernumberofCD14+cellsandreducedlevelofNGF,osteopontin,ITGA7,andNTF3geneexpressionwasidentifiedwhenHPLwasused.TheabilityofMSCs'osteogenicdifferentiationwashigherinHPL-supplementedmedium.Conclusion:DuringexpansionofMSCsusingHPLtheirdifferentiationabilitywaschangedwithaccompaniedgeneexpressionprofilechangesandwithproliferationabilitythatwascomparabletoFCS.

【 授权许可】

Unknown