Children with severe early childhood caries: streptococci genetic strains within carious and white spot lesions

Journal of Oral Microbiology

关键词: oral streptococci;    distribution of genotypic mutan streptococci strains;    Streptococcus mutans;    Streptococcus gordonii;    severe early childhood caries;   

DOI  :  

来源: Co-Action Publishing

【 摘 要 】

Backgroundandobjectives:Mutansstreptococci(MS)areoneofthemajormicrobiologicaldeterminantsofdentalcaries.TheobjectivesofthisstudyaretoidentifydistinctMSandnon-MSstreptococcistrainsthatarelocatedatcarioussitesandnon-cariousenamelsurfacesinchildrenwithsevereearlychildhoodcaries(S-ECC),andassessifcariogenicMSandnon-cariogenicstreptococcimightindependentlyexistasprimarybacterialstrainsondistinctsiteswithinthedentitionofindividualchildren.Design:Dentalplaquefromchildren(N=20;aged3–6)withS-ECCwascollectedfromcariouslesions(CLs),whitespotlesions(WSLs)andnon-cariousenamelsurfaces.Streptococcalisolates(N=10–20)fromeachsiteweresubjectedtopolymerasechainreaction(PCR)toidentifyMS,andarbitrarilyprimed-PCRforassignmentofgeneticstrains.Primarystrainswereidentifiedas≥50%ofthetotalisolatessurveyedatanysite.Inseveralcases,strainswerecharacterizedforacidurityusingATP-drivenbioluminescenceandsubjectedtoPCR-determinationofpotentialMSvirulenceproducts.Identificationofnon-MSwasdeterminedby16SrRNAgenesequencing.Results:Sixty-fourindependentMSornon-MSstreptococcalstrainswereidentified.Allchildrencontained1–6strains.Inmanypatients(N=11),singleprimaryMSstrainswereidentifiedthroughoutthedentition.Inotherpatients(N=4),primaryMSstrainswereidentifiedwithinCLsthatweredistinctfromprimarystrainsfoundonenamel.StreptococcusgordoniistrainswereidentifiedasprimarystrainsonenamelorWSLsinfourchildren,andingeneralwerelessaciduricthanMSstrains.Conclusions:ManychildrenwithS-ECCcontainedonlyasingleprimaryMSstrainthatwaspresentinbothcariousandnon-carioussites.Insomecases,MSandnon-cariogenicS.gordoniistrainswerefoundtoindependentlyexistasdominantstrainsatdifferentlocationswithinthedentitionofindividualchildren,andtheaciduricpotentialofthesestrainsmayinfluencesusceptibilityinthedevelopmentofCLs.Keywords:oralstreptococci;distributionofgenotypicmutans;streptococcalstrains;Streptococcusmutans;Streptococcusgordonii;severeearlychildhoodcaries(Published:29October2014)Citation:JournalofOralMicrobiology2014,6:25805-http://dx.doi.org/10.3402/jom.v6.25805

【 授权许可】

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