Production of PHB from Chicory Roots – Comparison of Three Cupriavidus necator Strains

Chemical and biochemical engineering quarterly

B. Maestro Madurga¹  C. Haas¹  V. Steinwandter¹  E. Diaz De Apodaca¹  T. T. Dietrich¹  M. Neureiter¹  M. Smerilli¹ 

关键词: Cichorium intybus;    fructose;    inulin;    by-product;    food residue;    pre-treatment;    hydrolysate;    batch fermentation;    Cupriavidus necator;    P3HB;    polymer characterisation;   

DOI  :  10.15255/CABEQ.2014.2250

来源: Croatian Society of Chemical Engineers

【 摘 要 】

Abstract Chicory roots from hydroponic salad cultivation are an abundant food residue in Navarra (Spain) that are underutilized to date. Aiming at a holistic utilization of resources, we report here the first process using chicory root hydrolysate for the production of poly([R]-3-hydroxybutyrate) (PHB). The polymer can be used for packaging material made for the locally produced vegetables. In the first step, we developed a pre-treatment process to obtain a hydrolysate, which contained 34 g L–1 sugars and 0.7 g L–1 total Kjeldahl nitrogen. This hydrolysate was used as fermentation substrate for three PHB-producing strains. Cupriavidus necator DSM 428 reached a dry biomass concentration of 11.3 g L–1 with a PHB content of 66 % in dry mass within 5 days. C. necator DSM 531 yielded 3.5 g L–1 dry biomass containing 46 % PHB within the same period. C. necator DSM 545 was superior over the other two in that 14.0 g L–1 of biomass containing 78 % PHB after only 3 days were obtained. These results show that even within the same species, the productivities on natural substrates are very different. The produced polymers were extracted using chloroform, and several thermo-physical parameters are in good accordance with published data. Overall, our holistic approach and the encouraging results prove that chicory roots are a viable fermentation substrate for PHB-production.

【 授权许可】

Unknown