Alkaline Protease Production by Solid State Fermentation on Polyurethane Foam

Chemical and biochemical engineering quarterly

W. Hui¹  Z. Aijun¹  H. Chen¹  L. Zuohu¹ 

关键词: Inert support;    solid state fermentation;    Alkaline protease;    PUF;   

DOI  :  

来源: Croatian Society of Chemical Engineers

【 摘 要 】

Abstract This paper investigated the process of solid state fermentation (SSF) using PUF (polyurethane foam) as inert solid support to produce alkaline protease. Maximal enzyme activity was 2185 U ml–1 at pH 9.0 incubation temperature 32 °C, inoculum amount of ρ = 1.0 %, nutrient solution 3.75 ml g–1 PUF, incubation time for 2 h and 15.0 m mol L–1 of added CaCl2. Under the same conditions, the yield of alkaline protease produced by SSF using PUF as support is higher than that by submerged fermentation (SMF).

【 授权许可】

Unknown