Isolation and partial characterization of a protease from Cucurbita ficifolia

FEBS Letters

Tapia, Guillermina¹  O'Reilly, Sybil¹  González, Gustavo¹  Curotto, Emilia¹ 

[1] Instituto de Química, Facultad de Ciencias Básicas y Matemáticas, Universidad Católica de Valparaiso, Casilla 4059, Valparaiso, Chile

关键词: Protease;    Alkaline protease;    Enzyme purification;    (Cucurbitaceae;    Cucurbita ficifolia);   

DOI  :  10.1016/0014-5793(89)80162-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A protease from the pulp of Cucurbita ficifolia was purified. Its molecular mass was estimated to be about 60 kDa. Its maximum activity is in the alkaline region against azocollagen as substrate. The enzyme is inhibited by phenylmethylsulphonyl fluoride but not by EDTA and iodoacetic acid.

【 授权许可】

Unknown