Gene expression alterations in doxorubicin resistant MCF7 breast cancer cell line

Genomics

Malek Zihlif¹  Shatha AbuHammad¹ 

关键词: MCF7;    Gene expression;    Doxorubicin resistance;   

DOI  :  10.1016/j.ygeno.2012.11.009

学科分类：医学（综合）

来源: Academic Press

【 摘 要 】

Manymolecularmechanismscontributetothedevelopmentofdoxorubicinresistanceanddifferentcancerscanexpresswideanddiversearraysofdrug-resistancegenes.TheaimofthisstudywastoidentifythechangesingeneexpressionassociatedwiththedevelopmentofdoxorubicinresistanceinMCF7breastcancercellline.ThedoxorubicinresistantMCF7celllinewasdevelopedbystepwiseselectionofMCF7cellsandwastestedusingtheMTTassay.Thealterationsingeneexpressionwereexaminedusingthereal-timebasedPCRarray.Thefindingsshowedanup-regulationofmanyphaseI/IImetabolizinggenes,specifically,theCYP1A1andtheCYP1A2thatwereup-regulatedby206-and96-foldrespectively.Drugeffluxpumpgeneswerealsoup-regulatedprofoundly.TOP2Awasstronglydown-regulatedby202-fold.Manyotherchangeswereobservedingenescrucialforcellcycle,apoptosisandDNArepair.Thefindingsofthisprojectimplythatthedevelopmentofdoxorubicinresistanceisamulti-factorialprocess.

【 授权许可】

Unknown   

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