Experimental identification of small non-coding regulatory RNAs in the opportunistic human pathogen Burkholderia cenocepacia J2315

Genomics

Christian G. Ramos²  Jorge H. Leitão¹  Paulo J.P. da Costa²  André M. Grilo² 

[1] Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade Técnica de Lisboa, Av. Rovisco Pais, Torre Sul, Piso 6, 1049-001, Lisboa, PortugalDepartment of Bioengineering, Instituto Superior Técnico, Universidade Técnica de Lisboa, Av. Rovisco Pais, Torre Sul, Piso 6, 1049-001, Lisboa, Portugal;Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Universidade Técnica de Lisboa, Av. Rovisco Pais, Torre Sul, Piso 6, 1049-001, Lisboa, Portugal

关键词: Burkholderia cenocepacia J2315;    Burkholderia cenocepacia J2315 sRNA;    Hfq;    Co-purification;    Target prediction;   

DOI  :  10.1016/j.ygeno.2012.10.006

学科分类：医学（综合）

来源: Academic Press

【 摘 要 】

Smallnon-codingregulatoryRNAs(sRNAs)playimportantrolesinregulatinggeneexpressionatthepost-transcriptionallevelandoftenrequiretheRNAchaperoneHfq.ThehumanopportunisticpathogenBurkholderiacenocepaciaJ2315encodestwodistinctRNAchaperones,HfqandHfq2.Thepresentworkdescribestheexperimentalidentificationandvalidationof24sRNAsfromB.cenocepaciaJ2315,basedontheco-purificationofsRNAswiththebacteriumHfqprotein,followedbyconversionintocDNA,cloning,computationalanalysisofsequencesandvalidationbyNorthernblotanalysis.ThesRNAsherereportedescapedidentificationbypreviousstudiesbasedontranscriptomicsorbioinformaticanalyses.Resultspresentedindicatethat3sRNAsareexclusivetobacteriaoftheBurkholderiacepaciacomplexandhavenohomologuesinotherbacteria,whiletheother21sharehomology,atdifferentextents,tosRNAsofotherbacterialspecies.

【 授权许可】

Unknown   

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