Pitx2 Distinguishes Subtypes of Terminally Differentiated Neurons in the Developing Mouse Neuroepithelium

Developmental Biology

Sally A. Camper¹  Donna M. Martin¹  Sharon E. Fox¹  Philip J. Gage¹  Jennifer M. Skidmore¹ 

[1] Departments of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, 48109

关键词: GABA;    nestin;    GAD67;    transcription factor;    cell fate determination;    proliferation;    differentiation;   

DOI  :  10.1006/dbio.2002.0835

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Pitx2,ahomeodomaintranscriptionfactor,isessentialfornormaldevelopmentofpituitary,eyes,heart,andteeth.Inthedevelopingmousebrain,Pitx2(Rieg,Ptx2,Otlx2,Brx1)mRNAisexpressedindiscreteregionsofthediencephalon,mesencephalon,andrhombencephalon.WhilepriorreportshaveprovidedanoverviewofthetemporalandregionalspecificityofPitx2mRNAexpressioninthebrain,theprecisecelltypesthatexpressPITX2arenotknown.Inthisstudy,weanalyzedPitx2mRNAandPITX2proteinexpressioninindividualcellsofthedevelopinge10.5–e14.5mouseCNSusingmultiplemarkersofcellularproliferationanddifferentiation.WeidentifiedPitx2expressioninnestin-positiveneuralprogenitorsandinpostmitotic,developingneurons.Inthediencephalon,PITX2isexpressedinneuronsofthezonalimitansintrathalamicaandmammillaryregionandinγ-aminobutyricacid(GABA)-producingneuronsofthezonaincerta.Inthemesencephalon,PITX2-labelednucleialsoappearindifferentiatedneurons,someofwhichareGABAergicanddestinedtooccupysuperiorcolliculus.OurresultssuggestthatPITX2expressioninpostmitoticneuronsmaycontributetodevelopmentofGABAergicandotherdifferentiatedneuronalphenotypes.

【 授权许可】

Unknown