Radar Is Required for the Establishment of Vascular Integrity in the Zebrafish

Developmental Biology

Christopher J. Hall¹  Kathryn E. Crosier¹  Maria Vega C. Flores¹  Philip S. Crosier¹  Alan J. Davidson¹ 

[1] Department of Molecular Medicine & Pathology, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag, 92019, Auckland, New Zealand

关键词: radar;    GDF6a;    BMP;    TGF-β;    zebrafish;    angiogenesis;    hypochord;    vasculogenesis;    morpholino;    microangiography;   

DOI  :  10.1006/dbio.2002.0794

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thepreciseassemblyofanintegratednetworkofbloodvesselsisessentialforthesurvivalofvertebrateembryos.However,theprocessesbywhichprimitiveendothelialcellsformmaturevesselscapableofsupplyingoxygenandnutrientstodevelopingtissuesremainincompletelyunderstood.Here,weproposearoleforRadar,oneofthezebrafishorthologuesofgdf6,inestablishingintegrityofthetrunkvasculatureinzebrafishembryos.Weshowthatradarexpressionisappropriatelyplaced,bothspatiallyandtemporally,toperformsucharole.Transcriptsforradararedetectedinthehypochordandtheprimitivegutendoderm.Thesetissuesintimatelyflankdevelopingaxialvesselsinthetrunkandhavebeenpreviouslyimplicatedintheregulationofvasculardevelopment.Morpholino-basedtargetedgeneknock-downhasgeneratedaRadar-specificloss-of-functionzebrafishmodel.Theseembryosdisplaynormalinitiationofvascularpatterningandcommencementofcirculation.However,byday2ofdevelopment,theintegrityoftheaxialvasculatureiscompromisedwithhemorrhagesandcirculationshort-circuitsthroughoutthedevelopingtrunk.Weshowthatthisaberrantvasculardevelopmentisspecifictoareductionoftheradargeneproduct.TheseresultssuggestthatRadarisinvolvedinasignalingpathwayrequiredforestablishingtheintegrityoftheaxialvesselsduringzebrafishdevelopment.

【 授权许可】

Unknown