Presence and Roles of Calcium Gradients along the Dorsal-Ventral Axis in Drosophila Embryos

Developmental Biology

Lionel F. Jaffe¹  Jill A. Kreiling¹  Robbert Créton¹ 

[1] Marine Biological Laboratory, Woods Hole, Massachusetts, 02543

关键词: pattern formation;    embryonic development;    signaling pathways;    dorsoventral axis;    [Ca2+];    TGF-β;    aequorin;    imaging photon detector;   

DOI  :  10.1006/dbio.1999.9542

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Dorsal-ventralspecificationoftheDrosophilaembryoismediatedbysignalingpathwayswhichhavebeenverywelldescribedingeneticterms.However,littleisknownaboutthephysiologyofDrosophiladevelopment.ByimagingpatternsoffreecytosoliccalciuminDrosophilaembryos,wefoundthatseveralcalciumgradientsaregeneratedalongthedorsal-ventralaxis.Themostpronouncedgradientisformedduringstage5,inwhichcalciumlevelsarehighdorsally.Manipulationofthestage5calciumgradientaffectsspecificationoftheamnioserosa,thedorsal-mostregionoftheembryo.Wefurthershowthatthiscalciumgradientisinhibitedinpipe,Toll,anddorsalmutants,butisunalteredindecapentaplegic(dpp)orpuntmutants,suggestingthatthestage5calciumgradientisformedbyasuppressionofventralcalciumconcentrations.Weconcludethatcalciumplaysaroleinspecificationofthedorsalembryonicregion.

【 授权许可】

Unknown