Developmental Biology | |
Constitutively Active Myosin Light Chain Kinase Alters Axon Guidance Decisions in Drosophila Embryos | |
Janice L Fritz1  Ananda K Seneviratne1  Mark F.A VanBerkum1  You-Seung Kim1  | |
[1] Department of Biological Sciences, Wayne State University, Detroit, Michigan, 48202 | |
关键词: Myosin; Myosin Light Chain Kinase; Calmodulin; motility; axon guidance; repulsion; Drosophila; | |
DOI : 10.1006/dbio.2002.0768 | |
学科分类:生物科学(综合) | |
来源: Academic Press | |
【 摘 要 】
ConventionalmyosinIIactivityprovidesthemotileforceforaxonoutgrowth,buttoachievedirectionalmovementduringaxonpathwayformation,myosinactivityshouldberegulatedbytheattractiveandrepulsiveguidancecuesthatguideanaxontoitstarget.Here,evidenceforthisregulationisobtainedbyusingaconstitutivelyactiveMyosinLightChainKinase(ctMLCK)toselectivelyelevatemyosinIIactivityinDrosophilaCNSneurons.ExpressionofctMLCKpan-neurallyorinprimarilypCC/MP2neuronscausestheseaxonstocrossthemidlineincorrectly.Thisoccurswithoutalteringcellfatesandissensitivetomutationsintheregulatorylightchains.TheseresultsconfirmtheimportanceofregulatingmyosinIIactivityduringaxonpathwayformation.MutationsinthemidlinerepulsiveligandSlit,oritsreceptorRoundabout,enhancethenumberofctMLCK-inducedcrossovers,butctMLCKexpressionalsopartiallyrescuescommissureformationincommissurelessmutants,whererepulsivesignalsremainhigh.OverexpressionofFrazzled,thereceptorformidlineattractiveNetrins,enhancesctMLCK-dependentcrossovers,butcrossoversaresuppressedwhenFrazzledactivityisreducedbyusingloss-of-functionmutations.TheseresultsconfirmthatproperpathwayformationrequirescarefulregulationofMLCKand/ormyosinIIactivityandsuggestthatregulationoccursindirectresponsetoattractiveandrepulsivecues.
【 授权许可】
Unknown
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