Multiple Cdk1 Inhibitory Kinases Regulate the Cell Cycle during Development

Developmental Biology

Walter F. Leise III¹  Paul R. Mueller¹ 

[1] Department of Biochemistry and Molecular Biology, Center for Molecular Oncology, Committees on Developmental Biology, Cancer Biology, and Genetics, University of Chicago, 924 E. 57th Street, Chicago, Illinois, 60637

关键词: Wee1;    Myt1;    Wee2;    Cdc2;    gastrulation;    cell cycle;    Xenopus;    cell division;    morphogenesis;    embryogenesis;   

DOI  :  10.1006/dbio.2002.0743

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

TheWeekinasesblockentryintomitosisbyphosphorylatingandinhibitingtheactivityofthemitoticcyclin-dependentkinase,Cdk1.WehavefoundthatthevariousXenopusWeekinaseshaveuniquetemporalandspatialpatternsofexpressionduringdevelopment.Inaddition,wehaveisolatedandcharacterizedanewWee1-likekinase,XenopusWee2.Bybothinvivoandinvitrotests,XenopusWee2functionsasaWee1-likekinase.ThepreviouslyisolatedWee1-likekinase,XenopusWee1,isexpressedonlyasmaternalgeneproduct.Incontrast,XenopusWee2ispredominantlyazygoticgeneproduct,whilethethirdWeekinase,XenopusMyt1,isbothamaternalandzygoticgeneproduct.ConcurrentwiththechanginglevelsoftheseCdkinhibitorykinases,thepatternofembryoniccelldivisionbecomesasynchronousandspatiallyrestrictedintheXenopusembryo.Interestingly,oncezygotictranscriptionbegins,XenopusWee2isexpressedinregionsoftheembryothataredevoidofmitoticcells,suchastheinvolutingmesoderm.Incontrast,XenopusMyt1isexpressedinregionsoftheembryothathavehighlevelsofproliferation,suchasthedevelopingneuraltissues.TheexistenceofmultipleWeekinasesmayhelpexplainhowdistinctpatternsofcelldivisionariseandareregulatedduringdevelopment.

【 授权许可】

Unknown