Sonic Hedgehog Regulates Otic Capsule Chondrogenesis and Inner Ear Development in the Mouse Embryo

Developmental Biology

H. Zhang¹  W. Liu¹  G. Li¹  J.S. Chien¹  C. Chiang¹  S. Raft¹  D.A. Frenz¹ 

[1] Department of Otolaryngology, Albert Einstein College of Medicine, Bronx, New York, 10461

关键词: Sonic hedgehog;    inner ear;    chondrogenesis;    morphogenesis;    oligonucleotides;    mutant mice;   

DOI  :  10.1006/dbio.2002.0733

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Developmentofthecartilaginouscapsuleoftheinnerearisdependentoninteractionsbetweenoticepitheliumanditssurroundingperioticmesenchyme.Duringthesetissueinteractions,factorsendogenoustotheoticepitheliuminfluencethedifferentiationoftheunderlyingperioticmesenchymetoformachondrifiedoticcapsule.WereportthelocalizationofSonichedgehog(Shh)proteinandexpressionoftheShhgeneinthetissuesofthedevelopingmouseinnerear.WedemonstrateinculturesofperioticmesenchymethatShhalonecannotinitiateoticcapsulechondrogenesis.However,whenShhisaddedtoculturedperioticmesenchymeeitherincombinationwithoticepitheliumoroticepithelial-derivedfibroblastgrowthfactor(FGF2),asignificantenhancementofchondrogenesisoccurs.AdditionofShhantisenseoligonucleotide(AS)toculturedperioticmesenchymewithaddedoticepitheliumdecreaseslevelsofendogenousShhandsuppressesthechondrogenicresponseofthemesenchymecells,whilesupplementationofShhAS-treatedcultureswithShhrescuesculturesfromchondrogenicinhibition.WedemonstratethatinactivationofShhbytargetedmutationproducesanomaliesinthedevelopinginnerearanditssurroundingcapsule.OurresultssupportaroleforShhasaregulatorofoticcapsuleformationandinnereardevelopmentduringmammalianembryogenesis.

【 授权许可】

Unknown