The Latent-TGFβ-Binding-Protein-1 (LTBP-1) Is Expressed in the Organizer and Regulates Nodal and Activin Signaling

Developmental Biology

Ignacio Muñoz-Sanjuán¹  Michael Heke¹  Daniel B. Rifkin¹  Curtis R. Altmann¹  Esther Bell¹  Ali H. Brivanlou¹  Chenbei Chang¹ 

[1] Laboratory of Molecular Embryology, The Rockefeller University, 1230 York Avenue, New York, New York, 10021

关键词: LTBP;    activin;    nodal;    Xenopus;    mesoderm;    induction;    morphogen;    organizer;   

DOI  :  10.1006/dbio.2002.0716

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

ThelatentTGF-βbindingproteins(LTBP)arebelievedtocontroltheavailabilityofTGF-βintheextracellularmilieu.TogaininsightintothepotentialrolesofLTBPinearlydevelopment,weisolatedtheXenopusLTBP-1(xLTBP-1)cDNA.ThecDNAencodesaproteinsimilartothemammalianLTBP-1inbothsizeanddomainstructure.Inaddition,wefoundanovellongerspliceisoformofxLTBP.TheRNAsforbothformsofxLTBPdisplayedtemporalregulationandtheshortertranscriptisexpressedmaternally.Bothtranscriptsalsodisplayspatialregulationandarefoundinthedorsalmesodermoftheorganizer.Inanimalcapexperiments,LTBP-1potentiatestheactivityofactivinandnodal.TheactivityofLTBP-1didnotappeartorequirecovalentassociationwithactivinastheadditionofmediumcontainingactivinandLTBP-1toanimalcapsenhancedtheactivineffect.TheseresultsindicatethatLTBP-1maybepartoftheregulatorysystemthatestablishesthethresholdofmorphogenactivityforactivinsandnodalsinthedorsalsideoftheembryoduringgastrulation.

【 授权许可】

Unknown