Fgf4 Positively Regulates scleraxis and Tenascin Expression in Chick Limb Tendons

Developmental Biology

Bernadette Schuler¹  Frédérique Edom-Vovard¹  Marie-Ange Bonnin¹  Marie-Aimée Teillet¹  Delphine Duprez¹ 

[1] Institut d'Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France (UMR 7128), 49 bis, avenue de la Belle Gabrielle, 94736, Nogent-sur-Marne, France

关键词: Fgf4;    Fgf8;    scleraxis;    tenascin;    tendons;    limb bud;    chick embryo;   

DOI  :  10.1006/dbio.2002.0707

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Invertebrates,tendonsconnectmusclestoskeletalelements.Surgicalexperimentsinthechickhaveunderlineddevelopmentalinteractionsbetweentendonsandmuscles.Initialformationoftendonsoccursautonomouslywithrespecttomuscle.However,furthertendondevelopmentrequiresthepresenceofmuscle.Themolecularsignalsinvolvedintheseinteractionsremainunknown.Inthechicklimb,Fgf4transcriptsarelocatedattheextremitiesofmuscles,wherethefuturetendonswillattach.Inthispaper,weanalysetheputativeroleofmuscle-Fgf4ontendondevelopment.Wehaveusedthreegeneraltendonmarkers,scleraxis,tenascin,andFgf8toanalysetheregulationofthesetendon-associatedmoleculesbyFgf4underdifferentexperimentalconditions.IntheabsenceofFgf4,inmusclelessandaneurallimbs,theexpressionofthethreetendon-associatedmolecules,scleraxis,tenascin,andFgf8,isdown-regulated.ExogenousimplantationofFgf4innormal,aneural,andmusclelesslimbsinducesscleraxisandtenascinexpressionbutnotthatofFgf8.TheseresultsindicatethatFgf4expressedinmuscleisrequiredforthemaintenanceofscleraxisandtenascinbutnotFgf8expressionintendons.

【 授权许可】

Unknown