Infertility of CD9-Deficient Mouse Eggs Is Reversed by Mouse CD9, Human CD9, or Mouse CD81; Polyadenylated mRNA Injection Developed for Molecular Analysis of Sperm–Egg Fusion

Developmental Biology

Shunichi Miyazaki¹  Shoji Oda¹  Akira Kudo¹  Keisuke Kaji¹ 

[1] Department of Life Science, Tokyo Institute of Technology, Yokohama, 226-8501, Japan

关键词: fertilization;    sperm–egg fusion;    polyadenylated mRNA injection;    CD9;    CD81;   

DOI  :  10.1006/dbio.2002.0694

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

CD9isamembraneproteinbelongingtothetetraspaninfamily.DespiteCD9'sbroadtissuedistribution,theonlyabnormalityobservedinCD9-deficientmicewasinfertilityoffemales,whichwasresponsibleforadefectinthesperm–eggfusionprocess.However,thefunctionofCD9insperm–eggfusionisnotclearatallbecausethetechniquetoanalyzetheactivityofmoleculesinsperm–eggfusionhasnotbeenestablished.WedemonstratedthattheexogenousmouseCD9,expressedbypolyadenylatedmRNAinjectionatthegerminal-vesiclestageoocytes,waspreciselylocalizedtotheeggplasmamembrane,andtheexpressionreversedtheinfertilityofCD9−/−eggs.Then,twoothertetraspanins,humanCD9andmouseCD81,overexpressedwiththistechniqueonCD9−/−eggsrestoredthefertilizationrateupto∼90and∼50%againstthatofwildtypeeggs,respectively.Moreover,inthepresenceofananti-mouseCD9mAb,whichblockssperm–eggfusion,expressionofhumanCD9ormouseCD81oneggsalsorescuedthefusibility.TheseresultssuggestedthathumanCD9playsacrucialroleinhumanfertilization,andmouseCD81hasthepotentialtocompensateforCD9functioninsperm–eggfusion.Inaddition,thepolyadenylatedmRNAinjectioniseffectiveformolecularanalysisofsperm–eggfusion.

【 授权许可】

Unknown