Purification and partial characterization of CD9 antigen of human platelets

FEBS Letters

Yatomi, Yutaka²  Higashihara, Masaaki²  Nakahara, Kazuhiko²  Kurokawa, Kiyoshi²  Takahata, Kyoya¹ 

[1] Kureha Chemical Industry Co. Ltd, Shinjuku-ku, Tokyo 160, Japan;First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 Japan

关键词: CD9;    Platelet;    N-terminal sequence;   

DOI  :  10.1016/0014-5793(90)80265-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CD9 antigen (p24) was purified from human platelets and partially characterized. The yield was 75 μg from 10 units of platelet concentrates. p24 (38 000 copies/platelet) has intramolecular disulfide bond(s) and, in SDS-PAGE, consists of major 24-kDa molecule and minor 26- to 31-kDa molecules. The N-terminal sequence of p24, PVKGOTKXIKYLLFGFNFIF, indicates that the protein has not previously been characterized and amino terminus (position 12–20) is hydrophobic.

【 授权许可】

Unknown