FGF Signaling Regulates Expression of Tbx2, Erm, Pea3, and Pax3 in the Early Nasal Region

Developmental Biology

Annette Neubüser¹  Nicole Firnberg¹ 

[1] Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030, Vienna, Austria

关键词: Tbx2;    Erm;    Pea3;    Pax7;    Pax3;    Fgf8;    craniofacial development;    patterning;    nasal placode;    olfactory placode;    chick;   

DOI  :  10.1006/dbio.2002.0696

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Fgf8isrequiredfornormaldevelopmentofthenasalregion.Here,wehaveusedacandidateapproachtoidentifygenesthatareinducedinchicknasalmesenchymeinresponsetoFGFsignaling.Usinganexplantculturesystem,weshowthatexpressionofthetranscriptionfactorsTbx2,Erm,Pea3,andPax3,butnotPax7,innasalmesenchymeisregulatedbyectodermalsignalsinastage-dependentmanner.UsingbeadssoakedinrecombinantFGFproteinandanFGFreceptorantagonist,wefurthermoredemonstratethatFGFsignalingisnecessaryandsufficientforexpressionofTbx2,Erm,Pea3,andPax3,buthasnoeffectonPax7expression.Wealsoshowthat,withinthenasalmesenchyme,competencetorespondtoFGFsignalingisinitiallywidespreadanduniformbutbecomesrestrictedtoregionsnormallyexposedtoFGFatlaterstagesofdevelopment,coincidentwithchangesinFGFreceptorexpression.Finally,weprovideevidencethatFGF8alsoregulatesErmandPea3expressioninthenasalplacodes.Together,theseresultsidentifyTbx2,Erm,Pea3,andPax3asdownstreamtargetsofFGFsignalinginthefacialareaandsuggestthatthesegenesmaymediatesomeoftheeffectsofFGF8duringdevelopmentofthenasalregion.

【 授权许可】

Unknown