A Novel Dictyostelium Gene Encoding Multiple Repeats of Adhesion Inhibitor-like Domains Has Effects on Cell–Cell and Cell–Substrate Adhesion

Developmental Biology

Timothy R. Varney¹  Jayne Dolman¹  Hoa N. Ho¹  Daphne D. Blumberg¹  Chere' Petty¹  Elisabeth Casademunt¹ 

[1] Department of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, Maryland, 21250

关键词: anti-adhesive;    development;    disintegrin;    ornatin;    cell adhesion;   

DOI  :  10.1006/dbio.2002.0569

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

TheDictyosteliumproteinAmpA(adhesionmodulationproteinA)isencodedbythegeneoriginallyidentifiedbytheD11cDNAclone.AmpAcontainsrepeateddomainshomologoustoavarietyofproteinsthatinfluencecelladhesion.Theproteinaccumulatesduringdevelopment,reachingamaximallevelatthefingerstage.MuchoftheAmpAproteinisfoundextracellularlyduringdevelopment,andinculminants,AmpAisfoundinassociationwithanterior-likecells.CharacterizationofanampA−straingeneratedbygenereplacementrevealsasignificantincreaseincell–cellclumpingwhencellsarestarvedinnonnutrientbuffersuspensions.DevelopingampA−cellsarealsomoreadhesivetotheunderlyingsubstrateandaredelayedindevelopmentalprogression,withtheseverityofthedelayincreasingascellsaregrowninthepresenceofbacteriaorontissueculturedishesratherthaninsuspensionculture.ReintroductionoftheampAgenerescuesthedevelopmentaldefectsofampA−cells;however,expressionofadditionalcopiesofthegeneinwild-typecellsresultsinmoreseveredevelopmentaldelaysanddecreasedclumpinginsuspensionculture.WeproposethattheAmpAproteinfunctionsasananti-adhesivetolimitcell–cellandcell–substrateadhesionduringdevelopmentandthusfacilitatescellmigrationduringmorphogenesis.

【 授权许可】

Unknown