The Zebrafish trilobite Gene Is Essential for Tangential Migration of Branchiomotor Neurons

Developmental Biology

Shin-ichi Higashijima¹  Hitoshi Okamoto¹  Stephanie Bingham¹  Anand Chandrasekhar¹ 

[1] Division of Biological Sciences, University of Missouri, Columbia, Missouri, 65211

关键词: zebrafish;    hindbrain;    motor neuron;    tangential;    radial;    neuronal migration;    gastrulation;    rhombomere;    green fluorescent protein;    time-lapse microscopy;   

DOI  :  10.1006/dbio.2001.0532

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Newbornneuronsmigrateextensivelyintheradialandtangentialdirectionstoorganizethedevelopingvertebratenervoussystem.Weshowherethatmutationsinzebrafishtrilobite(tri)thataffectgastrulation-associatedcellmovementsalsoeliminatetangentialmigrationofmotorneuronsinthehindbrain.Inthewild-typehindbrain,facial(nVII)andglossopharyngeal(nIX)motorneuronsareinducedinrhombomeres4and6,respectively,andmigratetangentiallyintor6andr7(nVII)andr7(nIX).Inallthreetriallelesexamined,althoughnormalnumbersofmotorneuronsareinduced,nVIImotorneuronsarefoundexclusivelyinr4,andnIX-likemotorneuronsarefoundexclusivelyinr6.Themigrationofotherneuronalandnonneuronalcelltypesisunaffectedintrimutants.Rhombomereformationandthedevelopmentofotherhindbrainneuronsarealsounaffectedintrimutants.Furthermore,tangentialneuronalmigrationoccursnormallyinthegastrulationmutantknypek,indicatingthatthetrilobiteneuronphenotypedoesnotarisenonspecificallyfromaberrantgastrulation-associatedmovements.Weconcludethattrilobitefunctionisspecificallyrequiredfortwotypesofcellmigrationthatoccuratdifferentstagesofzebrafishdevelopment.

【 授权许可】

Unknown