olig2 Is Required for Zebrafish Primary Motor Neuron and Oligodendrocyte Development

Developmental Biology

Hae-Chul Park¹  Bruce Appel¹  Joanna S. Richardson¹  Amit Mehta¹ 

[1] Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, 37235

关键词: zebrafish;    oligodendrocyte;    glia;    motor neuron;    spinal cord;    neurogenesis;    Hedgehog;    Olig;   

DOI  :  10.1006/dbio.2002.0738

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Oligodendrocytesareproducedfromthesameregionoftheventralspinalcordthatearliergeneratedmotorneuronsinbirdandrodentembryos.MotorneuronandoligodendrocyteprecursorcellsexpressOliggenes,whichencodebasichelix–loop–helixtranscriptionfactorsthatplayimportantrolesinthedevelopmentofbothmotorneuronsandoligodendrocytes.Wefoundthatoligodendrocytesdevelopsimilarlyinzebrafishembryos,inthattheyarisefromventralspinalcordandmigratetonewpositions.Developingprimarymotorneuronsandoligodendrocytesexpressolig2asdoneuralplatecellsthatgiverisetobothprimarymotorneuronsandoligodendrocytes.Lossofolig2functionpreventedprimarymotorneuronandoligodendrocytedevelopment,whereasolig2overexpressionpromotedformationofexcessprimarymotorneuronsandoligodendrocytes.WeprovidegeneticevidencethatHedgehogsignalingisrequiredforzebrafisholig2expressionandoligodendrocytedevelopment.However,olig2overexpressiondidnotpromoteprimarymotorneuronoroligodendrocytedevelopmentinembryoswithreducedHedgehogsignalingactivity.OnepossibilityconsistentwiththesedataisthatHedgehogsignaling,partlybyinducingolig2expression,specifiesneuralprecursorcellsthathavepotentialforprimarymotorneuronoroligodendrocytefate.

【 授权许可】

Unknown