Role of CtBP in Transcriptional Repression by the Drosophila giant Protein

Developmental Biology

Kevin Wright¹  David N. Arnosti¹  Paolo Struffi¹  Ling Qin¹  Brandon Pabst¹  Jelani Thomas¹  Bethany Strunk¹ 

[1] Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, 48824-1319

关键词: giant;    CtBP;    transcriptional repression;    Krüppel;    even-skipped;   

DOI  :  10.1006/dbio.2001.0454

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thegiantproteinisashort-rangetranscriptionalrepressorthatrefinestheexpressionpatternofgapandpair-rulegenesintheDrosophilablastodermembryo.Short-rangerepressorsincludingknirps,Krüppel,andsnailutilizetheCtBPcofactorforrepression,butitisnotknownwhetherafunctionalinteractionwithCtBPisageneralpropertyofallshort-rangerepressors.WestudiedgiantrepressionactivityinaCtBPmutantandfindthatthiscofactorisrequiredforgiantrepressionofsome,butnotall,genes.Whiletargetsofgiantsuchastheeven-skippedstripe2enhancerandasyntheticlacZreportershowclearderepressionintheCtBPmutant,anothergianttarget,thehunchbackgene,isexpressednormally.AmorecomplexsituationisseenwithregulationoftheKrüppelgene,inwhichoneenhancerisrepressedbygiantinaCtBP-dependentmanner,whileanotherisrepressedinaCtBP-independentmanner.TheseresultsdemonstratethatgiantcanrepressbothviaCtBP-dependentandCtBP-independentpathways,andthatpromotercontextiscriticalfordetermininggiant-CtBPfunctionalinteraction.Toinitiatemechanisticstudiesofthegiantrepressionactivity,wehaveidentifiedaminimalrepressiondomainwithingiantthatencompassesresidues89–205,includinganevolutionarilyconservedregionbearingaputativeCtBPbindingmotif.

【 授权许可】

Unknown