Developmental Biology | |
Zebrafish Early Macrophages Colonize Cephalic Mesenchyme and Developing Brain, Retina, and Epidermis through a M-CSF Receptor-Dependent Invasive Process | |
Bernard Thisse1  Philippe Herbomel1  Christine Thisse1  | |
[1] Unité de Génétique des Déficits Sensoriels, URA 1968 du CNRS, Département des Biotechnologies, Institut Pasteur, 25 rue du Dr Roux, 75724, Paris Cedex 15, France | |
关键词: macrophages; macrophage motility; embryogenesis; zebrafish; microglia; epidermis; apolipoprotein E; fms; M-CSF/CSF-1; | |
DOI : 10.1006/dbio.2001.0393 | |
学科分类:生物科学(综合) | |
来源: Academic Press | |
【 摘 要 】
Theoriginofresident(noninflammatory)macrophagesinvertebratetissuesisstillpoorlyunderstood.Inthezebrafishembryo,werecentlydescribedaspecificlineageofearlymacrophagesthatdifferentiateintheyolksacbeforetheonsetofbloodcirculation.Wenowshowthattheseearlymacrophagesspreadinthewholecephalicmesenchyme,andfromthereinvadeepithelialtissues:epidermis,retina,andbrain—especiallytheoptictectum.Inthepanthermutant,whichlacksafunctionalfms(M-CSFreceptor)gene,earlymacrophagesdifferentiateandbehaveapparentlynormallyintheyolksac,butthenfailtoinvadeembryonictissues.Ourvideorecordingsthendocumentforthefirsttimethebehaviorofmacrophagesintheinvadedtissues,revealingthestrikingpropensityofearlymacrophagesinepidermisandbraintowanderrestlesslyamongepithelialcells.Thisunexpectedbehaviorsuggeststhattissuemacrophagesmaybeconstantly“patrollingâ€forimmuneandpossiblyalsodevelopmentalandtrophicsurveillance.At60hpost-fertilization,allmacrophagesinthebrainandretinaundergoaspecificphenotypictransformation,into“early(amoeboid)microgliaâ€:theybecomemorehighlyendocytic,theydown-regulatetheL-plastingene,andabruptlystartexpressinghighlevelsofapolipoproteinE,awell-knownneurotrophiclipidcarrier.
【 授权许可】
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