Zebrafish Early Macrophages Colonize Cephalic Mesenchyme and Developing Brain, Retina, and Epidermis through a M-CSF Receptor-Dependent Invasive Process

Developmental Biology

Bernard Thisse¹  Philippe Herbomel¹  Christine Thisse¹ 

[1] Unité de Génétique des Déficits Sensoriels, URA 1968 du CNRS, Département des Biotechnologies, Institut Pasteur, 25 rue du Dr Roux, 75724, Paris Cedex 15, France

关键词: macrophages;    macrophage motility;    embryogenesis;    zebrafish;    microglia;    epidermis;    apolipoprotein E;    fms;    M-CSF/CSF-1;   

DOI  :  10.1006/dbio.2001.0393

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Theoriginofresident(noninflammatory)macrophagesinvertebratetissuesisstillpoorlyunderstood.Inthezebrafishembryo,werecentlydescribedaspecificlineageofearlymacrophagesthatdifferentiateintheyolksacbeforetheonsetofbloodcirculation.Wenowshowthattheseearlymacrophagesspreadinthewholecephalicmesenchyme,andfromthereinvadeepithelialtissues:epidermis,retina,andbrain—especiallytheoptictectum.Inthepanthermutant,whichlacksafunctionalfms(M-CSFreceptor)gene,earlymacrophagesdifferentiateandbehaveapparentlynormallyintheyolksac,butthenfailtoinvadeembryonictissues.Ourvideorecordingsthendocumentforthefirsttimethebehaviorofmacrophagesintheinvadedtissues,revealingthestrikingpropensityofearlymacrophagesinepidermisandbraintowanderrestlesslyamongepithelialcells.Thisunexpectedbehaviorsuggeststhattissuemacrophagesmaybeconstantly“patrolling”forimmuneandpossiblyalsodevelopmentalandtrophicsurveillance.At60hpost-fertilization,allmacrophagesinthebrainandretinaundergoaspecificphenotypictransformation,into“early(amoeboid)microglia”:theybecomemorehighlyendocytic,theydown-regulatetheL-plastingene,andabruptlystartexpressinghighlevelsofapolipoproteinE,awell-knownneurotrophiclipidcarrier.

【 授权许可】

Unknown