Toxicity Assessments of Chalcone and Some Synthetic Chalcone Analogues in a Zebrafish Model

Molecules

Ya-Ting Lee¹  Tsorng-Harn Fong²  Hui-Min Chen²  Chao-Yuan Chang¹  Yun-Hsin Wang¹  Ching-Yuh Chern³ 

[1]Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan

[2] E-Mails:

[3]Department of Anatomy, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan

[4] E-Mails:

[5]Department of Applied Chemistry, National Chia-Yi University, Chia-Yi 60004, Taiwan

关键词: chalcone;    embryogenesis;    muscle;    toxicity;    zebrafish;   

DOI  :  10.3390/molecules19010641

来源: mdpi

【 摘 要 】

The aim of this study was to investigate the in vivo toxicities of some novel synthetic chalcones. Chalcone and four chalcone analogues 1a–d were evaluated using zebrafish embryos following antibody staining to visualize their morphological changes and muscle fiber alignment. Results showed that embryos treated with 3'-hydroxychalcone (compound 1b) displayed a high percentage of muscle defects (96.6%), especially myofibril misalignment. Ultrastructural analysis revealed that compound 1b-treated embryos displayed many muscle defect phenotypes, including breakage and collapse of myofibrils, reduced cell numbers, and disorganized thick (myosin) and thin (actin) filaments. Taken together, our results provide in vivo evidence of the myotoxic effects of the synthesized chalcone analogues on developing zebrafish embryos.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.