Diverse Genes Expressed in Distinct Regions of the Trunk Epithelium Define a Monolayer Cellular Template for Construction of the Oikopleurid House

Developmental Biology

Torben Kallesøe¹  Fabio Spada¹  Eric M. Thompson¹ 

[1] Sars International Centre for Marine Molecular Biology, Bergen High Technology Centre, Thormøhlensgt. 55, N-5008, Bergen, Norway

关键词: Appendicularia;    urochordate;    oikosin;    nuclear architecture;    nanoplankton;    cDNA RDA;    gene amplification;   

DOI  :  10.1006/dbio.2001.0414

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thefilter-feedinghousesecretedbyurochordateAppendiculariansisamongthemostcomplexextracellularstructuresconstructedbyanyorganism.ThisstructureallowstheAppendiculariatoexploitawiderangeoffoodparticlesizes,includingnanoplanktonandsubmicrometercolloids,establishingthemasanimportantandabundantcomponentofmarinezooplanktoncommunitiesthroughouttheworld.Theoikoplasticepithelium,amonolayerofcellscoveringthetrunkoftheanimal,isresponsibleforsecretionofthehouse.Theepitheliumhasafixednumberofcells,organizedindistinctfields,characterizedbydefinedcellshapesandnuclearmorphologies.Certainstructuresinthehouseappeartobespatiallylinkedtothesedifferentfieldsofcells.UsingcDNArepresentationdifferenceanalysis(cDNARDA)onwholeanimalsattwodifferentdevelopmentalstagesseparatedbythemetamorphictailshiftevent,weisolatedfourfamiliesofgenes(oikosins)thatareexpressedonlyfromspecificsubregionsoftheoikoplasticepithelium.Themolecularpatternsdefinedbyoikosingeneexpressionestablishtheepitheliumasanidealandeasilyaccessiblemonolayercellulartemplateforexploringcoordinateregulationofgeneexpression,cell–cellinteractionsinvolvedinpatternformation,gene/genomeamplification,andtheroleoftemporalchangesinnucleararchitectureinregulatinggeneexpression.

【 授权许可】

Unknown