Patterning through Differential Endoreduplication in Epithelial Organogenesis of the Chordate, Oikopleura dioica

Developmental Biology

Eric M. Thompson¹  Philippe Ganot¹ 

[1] Sars International Centre for Marine Molecular Biology, Bergen High Technology Centre, Thormøhlensgt. 55, N-5008, Bergen, Norway

关键词: endocycle;    urochordate;    polyploidisation;    Appendicularia;    bilateral symmetry;   

DOI  :  10.1006/dbio.2002.0834

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thecontributionsthatcontrolofcellproliferationandcellgrowthmaketodevelopmentalregulationoforganandbodysizeremainpoorlyexplored,particularlywithrespecttoendocyclesinpolyploidtissues.TheepitheliumofthemarinechordateOikopleuradioicaiscomposedofafixednumberofcellsgroupedinterritoriesaccordingtogene-specificexpressionandnuclearsizesandshapes.Astheanimalgrows10-foldduringthelifecycle,epithelialcellsincreaseinsizedifferentiallyasafunctionoftheirspatialposition.Weshowthatthiscellularpatternreflecteddifferencesinploidylevelsrangingfrom34to1300C.Thediverseploidylevelsindefinedcellularfieldsresultedbothfromdifferenttimingofentryintoendocyclesandfromcell-specificregulationofendocyclelengths.Throughoutthelifecycle,differentialcellsizeandploidyincreaseswereaccompaniedbyfield-specificprofilesofprogressivereductionsinG-phaseduration.Endocycleswereasynchronousamongcellsofagivenepithelialterritory,butattheresolutionofindividualcells,bothDNAreplicationtimingandploidylevelswerebilaterallysymmetric.Thetransparent,accessible,oikoplasticepitheliumisamodelofchoiceforthestudyofendoreduplicationinthecontextofpatternformationandgrowth.

【 授权许可】

Unknown