cGMP Enhances the Sonic Hedgehog Response in Neural Plate Cells

Developmental Biology

Christie P. Robertson¹  Sarah M. Gibbs¹  Henk Roelink¹ 

[1] Department of Biological Structure, Program in Neurobiology and Behavior, Center for Developmental Biology, Box 357420, University of Washington, Seattle, Washington, 98195

关键词: Sonic hedgehog;    chick;    neural;    patterning;    dorsoventral;    cGMP;    protein kinase;    nitric oxide;    natriuretic peptide;    guanylate cyclase;   

DOI  :  10.1006/dbio.2001.0392

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Theelaborationofdistinctcelltypesduringdevelopmentisdependentonasmallnumberofinductivemolecules.AmongtheseinducersisSonichedgehog(Shh),which,incombinationwithotherfactors,patternsthedorsoventral(DV)axisofthenervoussystem.Theresponseofacellisdependentinpartonitscomplementofcyclicnucleotides.cAMPantagonizesShhsignaling,andweexaminedtheinfluenceofcGMPontheShhresponse.CellsinchickneuralplateexplantsrespondtoShhbydifferentiatingintoventralneural-celltypes.Exposureofintermediate-zoneexplantstocGMPanalogsenhancedtheirresponsetoShhinadose-dependentmanner.TheShhresponsewasalsoenhancedindorsal-zoneexplantsexposedtochicknatriureticpeptide(chNP),whichstimulatescGMPproductionbymembrane-boundguanylatecyclase(mGC).AdditionofchNPtointermediate-zoneexplantsdidnotenhancetheShhresponse,consistentwithareportedlackofmGCinthisregionoftheneuraltube.Finally,thepresenceofanitricoxide(NO)-sensitiveguanylatecyclase(GC)wasestablishedbydemonstratingcGMPimmunoreactivityinneuraltissuefollowingNOstimulationofwholechickembryos.IntracellularlevelsofcGMPandcAMPmaythusprovideamechanismthroughwhichotherfactorsmodulatetheShhresponseduringneuraldevelopment.

【 授权许可】

Unknown