Cell Death and Neuronal Replacement during Formation of the Avian Ciliary Ganglion

Developmental Biology

Vivian M. Lee¹  Rae Nishi¹  Gregory G. Smiley¹ 

[1] Department of Cell & Developmental Biology, L-215, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, Oregon, 97201

关键词: parasympathetic;    apoptosis;    Hu;    TUNEL;    cranial neural crest;    peripheral nervous system;    ciliary neurotrophic factor;   

DOI  :  10.1006/dbio.2001.0236

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Programmedcelldeathisaprominentfeatureofembryonicdevelopmentandisessentialinmatchingthenumberofneuronstothetargettissuesthatareinnervated.Althoughadecreaseinneuronalnumberwhichcoincideswithperipheralsynaptogenesishasbeenwelldocumentedintheavianciliaryganglion,ithasnotbeenclearwhethercelldeathalsooccursearlier.WeobservedTUNEL-positiveneuronsasearlyasstage24,withalargepeakatstage29.Thiscelldeathatstage29wasfollowedbyastatisticallysignificant(P<0.0001)decreaseintotalneuronnumberatstage31.Thetotalnumberofneuronswasrecoveredbystage33/34.Thissuggestedthatdyingneuronswerereplacedbynewneurons.Thisreplacementprocessdidnotinvolveproliferationbecausebromodeoxyuridineappliedatstages29and31wasunabletolabelneuronsharvestedatstage33/34.Thepeakofcelldeathatstage29wasincreased2.3-foldbyremovaloftheopticvesicleandwasreducedby50%whenchCNTFwasoverexpressed.Takentogether,theseresultssuggestthattheregulationofneuronnumberintheciliaryganglionisadynamicprocessinvolvingbothcelldeathandneuralreplacementfrompostmitoticprecursorspriortodifferentiationandinnervationoftargettissues.

【 授权许可】

Unknown