Pannier is a Transcriptional Target and Partner of Tinman during Drosophila Cardiogenesis

Developmental Biology

Nancy Fossett¹  Young Ho Kim¹  Robert A. Schulz¹  Cheol Yong Choi¹  Kathleen Gajewski¹  Yongsok Kim¹  Qian Zhang¹  Anh Dang¹ 

[1] Department of Biochemistry and Molecular Biology, Graduate Program in Genes & Development, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, 77030

关键词: cardiogenic factors;    D-MEF2;    heart;    Pannier;    protein interactions;    Tinman;    transcriptional enhancer;   

DOI  :  10.1006/dbio.2001.0220

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

DuringDrosophilaembryogenesis,thehomeoboxgenetinmanisexpressedinthedorsalmesodermwhereitfunctionsinthespecificationofprecursorcellsoftheheart,visceral,anddorsalbodywallmuscles.TheGATAfactorgenepannierissimilarlyexpressedinthedorsal-mostpartofthemesodermwhereitisrequiredfortheformationofthecardialcelllineage.Despitetheseoverlappingexpressionandfunctionalproperties,potentialgeneticandmolecularinteractionsbetweenthetwogenesremainlargelyunexplored.Here,weshowthatpannierisadirecttranscriptionaltargetofTinmanintheheart-formingregion.Theresultingcoexpressionofthetwofactorsallowsthemtofunctioncombinatoriallyintheregulationofcardiacgeneexpression,andaphysicalinteractionoftheproteinshasbeendemonstratedinculturedcells.WealsodefinefunctionaldomainsofTinmanandPannierthatarerequiredfortheirsynergisticactivationoftheD-mef2differentiationgeneinvivo.Together,theseresultsprovideimportantinsightsintothegeneticmechanismscontrollingheartformationintheDrosophilamodelsystem.

【 授权许可】

Unknown