A clonal study of the reversible inhibition of muscle differentiation by the halogenated thymidine analog 5-bromodeoxyuridine☆

Developmental Biology

Elizabeth J.H. Hartline¹  John R. Coleman¹  Annette W. Coleman¹ 

[1] Brown University, Providence, Rhode Island 02912 USA

关键词: RNase;    ribonuclease;    DNase;    deoxyribonuclease;    PTA;    phosphotungstic acid;    FUdR;    5-fluorodeoxyuridine;    ClUdR;    5-chlorodeoxyuridine;    BUdR;    5-bromodeoxyuridine;    TdR;    thymidine;    BCdR;    5-bromodeoxycytidine;    IUdR;    5-iododeoxyuridine;    BUR;    5-bromouridine;    BU;    5-bromouracil;    OHMeUdR;    5-hydroxymethyldeoxyuridine;    2;    6-DAPdR;    2;    6-diaminopurine deoxyriboside;    5-MeCdR;    5-methyldeoxycytidine;    T;    thymine;    CdR;    deoxycytidine;    UdR;    deoxyuridine;    UR;    uridine;    U;    uracil;    TR;    thymine riboside;    AdR;    deoxyadenosine;    3-MeCdR;    3-methyldeoxycytidine;   

DOI  :  10.1016/0012-1606(69)90036-0

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thethymidineanalog5-bromodeoxyuridine(BUdR)iswellknownforitsmutageniceffectswhenincorporatedinDNA.Similarly,BUdRhasbeenreportedbyseveralworkerstoinhibitcytodifferentiationwhenappliedduringthecellproliferativephase.Wehaveobservedthatrelativelylowlevels(ca10−6M)ofBUdR,IUdR,ClUdR,andBCdRallcauseagradualchangeincellmorphology,andinhibitthefusionofcellstoformsyncytiainmuscleclones,butdonotinterferewithproliferationofmononuclearcells.Theinhibitionofdifferentiationcanbereversedbyfurthercelldivisionintheabsenceoftheanalog.Afterseveraldays,thisresultsinclonalcultureswhichareindistinguishablefromuntreatedcontrolsbothintermsoftotalnumberofclonesandoftheproportionofcloneswhicharemyogenic;thusthedrugsarenotactingeitherbycellselectionormutagenesis.BUdRisincorporatedintoDNAinplaceofthymine,itseffectondifferentiationcanbecompetitivelypreventedbysimultaneousinclusioninthemediumofTdRorcompoundsreadilyconvertibletoTdR,andithasnodetectableeffectonpostmitoticcells(musclefibers).AlthoughthissuggeststhatBUdRexertsitseffectondifferentiationfromasiteintheDNA,aneffectoncellmetabolismatsomeotherlevelcannotberuledout.

【 授权许可】

Unknown