Requirement for RNA and protein synthesis for induced regression of the tadpole tail in organ culture

Developmental Biology

J.R. Tata¹ 

[1]National Institute for Medical Research, Mill Hill, London, England

关键词: T3;    3;    5;    3′-triiodo-l;    RNA;    ribonucleic acid;    DNA;    deoxyribonucleic acid;    Tris;    2-amino-2-hydroxymethyl-1;    3-propanediol;   

DOI  :  10.1016/0012-1606(66)90050-9

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

AsimplemethodformaintainingthetailofRanatemporariatadpolesforupto8daysinorganculturehasbeendescribed.Regressionoftheisolatedtailwasinducedbytheadditionoftriiodothyroninetotheculturemedium.Therateofregressionandtheincreaseinactivityofsomehydrolyticenzymeswerecomparabletothoseobservedinthetailsofintacttadpolesundergoingmetamorphosis.CulturedtailsarecapableofsynthesizingRNAandproteinasseenfromtheincorporationofH3-labeleduridineandC14-labeledaminoacids.Hormone-inducedregressionwasaccompaniedbyanacceleratedsynthesisofbothRNAandprotein.ActinomycinD,puromycin,andcycloheximideabolishedregressionandincreaseinhydrolaseactivityintheisolatedtailsinducedbytriiodothyronine.Underconditionsinwhichregressionwasinhibited,actinomycinDandcycloheximideblockedtheincorporationofH3-uridineintoRNA,andpuromycinandcycloheximidethatofC14-aminoacidsintoprotein.AcontinuousgenerationofRNAandproteinisassociatedwiththehormone-inducedregressionoftheisolatedtadpoletail.

【 授权许可】

Unknown