Thyroid hormone increases transcription of GA‐binding protein/nuclear respiratory factor‐2 α‐subunit in rat liver

FEBS Letters

Rodrı́guez-Peña, Angeles¹  Escrivá, Héctor¹  Vallejo, Carmen G¹  Handler, Ana C¹ 

[1]Instituto de Investigaciones Biomédicas ‘Alberto Sols’ (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain

关键词: Mitochondria;    Thyroid hormone;    Nuclear respiratory factor;    GA-binding protein/nuclear respiratory factor-2;    Transcriptional regulation;    ATP synthase β-subunit;    GABP;    GA-binding protein;    TH;    thyroid hormone;    T3;    triiodothyronine;    NRF;    nuclear respiratory factor;    Tfam;    mitochondrial transcription factor A;   

DOI  :  10.1016/S0014-5793(02)02389-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thyroid hormone (TH) regulates mitochondrial respiratory rate by activating coordinated transcription in the nucleus and mitochondria. Whereas TH activates transcription of mitochondrial genes directly, the activation of nuclear-encoded mitochondrial genes is probably executed by indirect unknown mechanisms. Nuclear respiratory factors (NRF)-1 and GA-binding protein (BP)/NRF-2 may function as transacting genes, but regulation of these genes by TH is not demonstrated. We show that TH administration to hypothyroid rats promptly increases GABP/NRF-2 α-subunit mRNA levels in the liver, without significant changes in β, γ subunits. In run-on and time-course experiments, the transcription rate and protein levels increased three-fold in response to TH, indicating GABP/NRF-2 transcriptional regulation. The results also support the notion that ATP synthase β-subunit is regulated by TH through the indirect activation of GABP/NRF-2.

【 授权许可】

Unknown