Incorporation of C14-l-leucine into protein by cell-free systems from sea urchin embryos at different stages of development☆

Developmental Biology

Åsa Bergstrand¹  Tore Hultin¹ 

[1] The Wenner-Gren Institute, Stockholm, Sweden

关键词: ATP;    adenosine triphosphate;    PEP;    phosphoenol pyruvate;    RNA;    ribonucleic acid;    RNAse;    ribonuclease;    RNP-particles;    ribonucleoprotein particles;    TCA;    Trichloroacetic acid;    tris;    tris(hydroxymethyl)-aminomethane;   

DOI  :  10.1016/0012-1606(60)90016-6

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Inthepresenceofadenosinetriphosphateandphosphoenolpyruvate,C14-l-leucinewasincorporatedintoproteinbyhomogenatesofPsammechinuseggsandembryos.Theincorporationwasinhibitedbyribonuclease.Particularlyinunfertilizedeggs,aconsiderablepartoftheincorporationactivitywaslocalizedincomponentsofhomogenatessedimentingat12000g.Inhomogenatespreparedinstronghypotonicity,lessoftheincorporationactivitywassedimentable.Whenhomogenateswerepreparedinparallelfromunfertilizedandfertilizedeggs,thehomogenatesoffertilizedeggsshowedasignificantlyhigherincorporationactivity.Theleucine-incorporatingactivityofthehomogenates,asmeasuredundertheseexperimentalconditions,rapidlyincreasedduringtheearlystagesofdevelopment.Theincreasebegansoonafterfertilizationandculminatedinthelateblastulastage.Theincreasewasnotessentiallyduetomoreefficientaminoacidactivation;itwasequallyobviousinincorporationsystemsfortifiedwithlivercellsapasasourceofactivatingenzymes.Theincreaseisprobablyattributabletoahighercontentofactiveribonucleoproteinparticlesintheembryos,sinceitwasalsoobservedinincorporationexperimentswithlivercellsapcombinedwithpartiallypurifiedribonucleoproteinparticlesfromtheembryos.

【 授权许可】

Unknown